Figure 4.

Generation of neutralizing antibodies for Npnt. (A) Cross‐reactivity of anti‐Npnt‐FD antibody to plates coated with Npnt, fibronectin, or GRGDS peptide‐coupled with BSA (1 μg·mL⁻¹) by direct ELISA. (B) Specificity of anti‐Npnt‐FD antibody by western blotting. (C, D) NIH3T3 cells were allowed to adhere to 96‐well plates precoated with full‐length of Npnt or Npnt‐Link (C), fibronectin, or vitronectin (D) in the presence of indicated concentrations of anti‐Npnt‐FD antibody. Normal rabbit IgG was used as a control antibody. Data are presented as mean ± SEM. Data are representative of three or more independent experiments with similar results. *P < 0.05, anti‐Npnt‐FD antibody versus control antibody (Student's t‐test).