Figure 5.

Individual PE35 and PPE68 proteins reduce the number of double‐positive CD4⁺ cells. Splenocytes were restimulated with 10 µg·mL⁻¹ of recombinant proteins in culture for 8 h in the presence of GolgiPlug/GolgiStop solution (BD). Cells were washed and stained for intracellular IFN‐γ and IL‐2. (A) Individual PE35 and PPE68 proteins reduce the number CD4⁺ cells double‐positive for IFN‐γ and IL‐2. However, the numbers of CD8⁺ double‐positive for both were found to be unchanged. (B) Dot plots are the representative of experiments with four mice in each group. Statistical significance was determined by one‐way ANOVA, and data are presented as mean ± SD. P value < 0.05 was considered statistically significant.