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. 2019 Dec 10;10(1):56–69. doi: 10.1002/2211-5463.12745

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© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of st6gal1 knockdown on cell fate. (A) Co‐immunostaining of Pax7 (green) and MyoD (red) in C2C12‐sh‐Mock and C2C12‐sh‐Cl2 cells during proliferation. The exposure times were identical for a same staining between the two cell types (1000 ms for Pax7‐FITC and 500 ms for MyoD‐DsRed); scale bar: 200 µm. (B) Percentages of Pax7⁺/MyoD⁻, Pax7⁺/MyoD⁺, and Pax7⁻/MyoD⁺ cell populations during proliferation. Percentages are the mean of counting on seven fields by condition. Experiments have been done three times (n = 3). Means ± SEMs are shown (two‐tailed t‐test, with a significant level of ***P < 0.001).