Table 2.

Classification of sialylated and/or fucosylated N‐glycans identified by MS/MS spectrometry. The identified glycans are classified according to their structure (oligomannosylated, neutral complex or sialylated complex glycans) and to their sialylated level (mono‐, bi‐, tri‐ or tetra‐sialylated). Mass spectrometry experiment has been done one time (n = 1). Cells of three independent cultures have been pooled before N‐glycans extraction and analyses.

		Mass (m/z)	C2C12‐sh‐Mock			C2C12‐sh‐Cl2
			Proliferation	Differentiation		Proliferation	Differentiation
				120 h MT	120 h RC + MB		120 h MT	120 h RC + MB
Oligomannosylated glycans		1579	9.89	1.50	7.16	7.63	2.41	9.52
		1783	6.37	1.46	7.53	9.40	3.05	16.31
		1988	3.83	0.47	3.58	4.39	0.87	6.74
		2192	3.74	0.55	4.44	4.76	1.09	8.19
		2395	3.57	0.49	4.47	3.22	0.00	7.46
		Total	27.40	4.47	27.18	29.41	7.43	48.22
Neutral complex glycans		2519	3.08	0.00	2.79	3.45	0.00	3.67
		2693	3.11	0.00	3.54	4.01	0.00	4.48
		2839	3.40	0.00	3.38	0.00	0.00	0.00
		Total	9.59	0.00	9.71	7.46	0.00	8.15
Sialylated complex glycans	Mono‐sialylated	2605	4.98	4.11	5.25	4.04	6.45	7.64
		3053	0.00	0.00	1.02	0.00	0.00	1.17
		2778	6.87	11.64	6.26	5.94	11.06	3.72
		2822	3.48	8.48	2.77	3.54	7.66	0.00
		3023	0.00	2.56	1.91	0.00	0.00	0.00
		3228	2.02	3.48	5.84	1.73	2.89	1.89
		Total	17.35	30.26	23.04	15.26	28.06	14.42
	Bi‐sialylated	2792	13.70	30.91	11.92	13.40	23.52	10.07
		3037	0.00	2.80	0.00	1.71	0.00	0.00
		3242	4.09	8.56	2.98	4.44	5.74	2.08
		2966	2.53	2.06	2.55	2.29	3.50	4.68
		Total	20.31	44.33	17.45	21.84	32.76	16.83
	Tri‐sia	3604	20.14	16.79	16.14	21.43	26.13	9.60
	Tetra‐sia	3966	5.20	4.15	6.47	4.59	5.62	2.78
	Total		63.01	95.53	63.11	63.13	92.57	43.62