Table 1. Primers used in pathogen detection, target genes amplified and size of the amplified product for each pathogen using real time PCR.
| Pathogen | Region amplified | Primer Forward (5′-3′) | Primer Reverse (5′-3′) | Final [primer] (µM) | PCR product (bp) |
|---|---|---|---|---|---|
| Ehrlichia/ Anaplasma spp. | 16S rRNA | GCAAGCYTAACACATGCAAGTCG | CTACTAGGTAGATTCCTAYGCATTACTCACC | 0.5 | 102a) |
| Piroplasmid | 18S rRNA | GACGATCAGATACCGTCGTAGTCC | CAGAACCCAAAGACTTTGATTTCTCTC | 0.3 | 114a) |
| Rickettsia spp. | ITS1 | GCTCGATTGRTTTACTTTGCTGTGAG | CATGCTATAACCACCAAGCTAGCAATAC | 0.5/0.3 | 300a) |
| Bartonella spp. | ITS1 | AGATGATGATCCCAAGCCTTCTG | CCTCCGACCTCACGCTTATCA | 0.3 | 180a) |
a) Targeted size could vary depending on the species.