Figure 3.

USP37 promotes cancer cell migration. (A) Immunoblotting of USP37, SNAI1, and β-actin in HEK293T cells transfected with USP37 siRNA or a scrambled control (NC). (B) Immunoblotting of FLAG-USP37, SNAI1, and β-actin in HEK293T cells transfected with SFB-tagged GFP, USP37, or USP37^C350S. (C) Immunoblotting of USP37, SNAI1, and β-actin in SUM159 and HCT116 cells transfected with USP37 siRNA or a scrambled control (NC). (D) Immunoblotting of FLAG-USP37, SNAI1, and β-actin in SUM159 and HCT116 cells transfected with SFB-tagged GFP, USP37, or USP37^C350S. (E) Immunoblotting of USP37, SNAI1, E-cadherin, vimentin, and β-actin in HEK293T cells transduced with RFP, USP37, or USP37^C350S. (F) Transwell migration assays of SUM159 cells transfected with SFB-tagged GFP, USP37, or USP37^C350S. (G) Transwell migration assays of HCT116 cells transfected with SFB-tagged GFP, USP37, or USP37^C350S. (H) Immunoblotting of USP37, SNAI1, and β-actin in HCT116 cells transduced with USP37 shRNA or scramble shRNA (NC). (I) Transwell migration assays of HCT116 cells transduced with scrambled shRNA or USP37 shRNA. (J) Growth curves of HCT116 cells transduced with scrambled shRNA or USP37 shRNA. n = 3 biological replicates. Error bars in (F), (G), (I), and (J) are s.e.m. P values were calculated from a two-tailed t-test.