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. 2019 Dec 1;9(12):2730–2748.

Figure 3.

Figure 3

Akr1b8 Knockout in mice promoted hepatocyte damage and Liver inflammation. A. Changes of serum ALT level in WT or Akr1b8-KO mice with or without DEN administration for 1, 6, 9 and 12 months, respectively. B. Western blot analysis of p-H2AX level in livers of WT and Akr1b8-KO mice 6 and 9 months after DEN injection. C. IHC of CD3 in the none-tumor liver tissues of WT and Akr1b8-KO mice 6 and 9 months after DEN injection. The scale bar is 100 μm. Average numbers of CD3 positive stained cells in five high power fields were calculated and the data represent means ± s.d. (n=5). D. Changes of serum IL-6 and TNF-α levels in WT and Akr1b8-KO mice 6 and 9 months after DEN injection. E. F4/80 IHC staining of macrophages infiltration in the livers of WT and Akr1b8-KO mice after 6-month DEN treatment. Average numbers of F4/80 positive stained cells in five fields were calculated and the data represent means ± s.d. (n=5). The scale bar is 100 μm. The arrows indicated positive staining. Statistical significance was determined with Student t two-tailed test. *indicated P<0.05. **indicated P<0.01. ***indicated P<0.001.