Figure 2.

SORBS2 overexpression inhibits HCC cell migration, invasion, and EMT. (A) SMMC-7721 and HCC-LM3 cells were infected with SORBS2-overexpressing lentivirus or controllentivirus (indicated as “Vector”). SORBS2 protein expression was determined using western blotting. (B) Cell proliferation at different time points was assessed by the MTT assay. Representative cell migration (C) and invasion (D) patterns of SMMC-7721 and HCC-LM3 cells, as determined by Transwell migration and invasion assays. Scale bar, 100 μm. (E) mRNA levels of E-cadherin, vimentin, Slug and Snail were determined by qRT-PCR. (F) Protein amounts of E-cadherin, Vimentin, Slug, and Snail were evaluated by western blotting. β-actin protein levels served as an internal control. *P < 0.05, **P < 0.01.