Figure 6.

SORBS2 is inversely related to MEF2D in HCC. A. A diagram of the promoter region of the SORBS2 gene and putative MEF2D-binding sites. pGL3-enhancer reporter vectors with or without the putative MEF2D binding sites are illustrated below. B. Western blotting analysis of MEF2D expression in four HCC cell lines. C. qRT-PCR and western blotting analyses of SORBS2 and MEF2D expression in SMMC-7721 and HCC-LM3 cells with MEF2D knockdown and in PLC or Huh7 cells with MEF2D overexpression. D. The relative luciferase activity of plasmids with different versions of the SORBS2 promoter was detected in MEF2D knockdown HCC-LM3 cells and MEF2D-overexpressing Huh7 cells. E. The ChIP assay was performed to detect the binding of MEF2D to the SORBS2 promoter. The IgG-treated and blank groups were regarded as negative controls, whereas the input fraction as a positive control. F. MEF2D mRNA levels in 12 pairs of HCC tissue samples and normal tissue samples. G. Pearson analysis of the correlation between SORBS2 and MEF2D mRNA levels in HCC tissue samples. *P < 0.05, **P < 0.01.