Figure 3.

Axl enhances YAP activity. A. H1299 cells were transiently transfected with TEAD/YAP-responsive luciferase plasmid (8xGTIIC-luc) and YAP expression plasmid and/or Axl expression plasmid. 48 hours after transfection, luciferase activity was determined. B. H1299 cells were transfected with TEAD/YAP-responsive luciferase plasmid (8xGTIIC-luc) with/without YAP expression plasmid. 48 hours after transfection, the cells were treated or untreated with GAS6 for 1 hour, and subjected to luciferase assay. C. Flag-YAP and/or His-tagged Axl were stably expressed in BT549 cells, and they were cultured in soft agar for 3 weeks. The numbers of colonies were counted and shown as the bar graph (n = 3, the left graph). Protein expression was confirmed by Western blot analysis (the right panels).