Figure 3.

Characterization of acoustofluidic chip exosome isolation. A–F: Particle size distributions of original samples of cell-free (A), Oasis-collected (B), and whole (C) and acoustofluidic-isolated cell-free (D), Oasis-collected (E), and whole (F) saliva. The acoustofluidic-isolated samples show low concentrations of particles bigger than 200 nm compared with the original samples. Dashed green lines show the 150 nm position on the x axis of the size distribution results. G and H: A transmission electron microscopy image demonstrates an isolated, whole saliva sample that contains fewer contaminants (G) than the original sample (H). Particles with exosomal morphology are tagged by red arrowheads. Boxed areas in top panels are shown at higher magnification in the lower panels. I: Western blot analysis for an exosomal protein biomarker. Only isolated exosome products demonstrate exosomal biomarkers with counts similar to the original sample. Scale bars: 500 nm (G and H, upper panels); 200 nm (G and H, lower panels).