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. 2020 Jan;22(1):50–59. doi: 10.1016/j.jmoldx.2019.08.004

Figure 4.

Figure 4

Comparison of yields of exosomes from acoustofluidic separation (AFS) technology and differential centrifugation. A: DNA samples extracted from cell-free saliva (CFS), different acoustofluidic isolated fractions, and exosomes from differential ultracentrifugation (UC) were tested with digital droplet RT-PCR assays for piR014923 and miR148a, which were reported to be predominately located in salivary exosomes. The pink lines show the threshold of positive droplets detection at 2000 amplitude. B: The yield (expressed as a relative fold difference) of individual small RNA in isolated salivary exosomes with acoustofluidic separation (AFS-Exo) technology and differential ultracentrifugation (UC-Exo) method. *P < 0.05 (t-test). a.u., arbitrary units; CF, cell debris fraction; Exo, exosome; MV, microvesicle.