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. 2020 Jan;22(1):50–59. doi: 10.1016/j.jmoldx.2019.08.004

Figure 5.

Figure 5

Detection of HPV16 DNA in acoustofluidic-isolated salivary exosomes of HPV16-positive oropharyngeal cancer (OPC) patients. A: Distribution of HPV16 DNA in exosomes (Exo) and microvesicles (MV) isolated from saliva of HPV16+ OPC by acoustofluidic technology. Representative results from two HPV16 exosome–positive patients (P1 and P6). Droplet digital PCR (ddPCR) HPV16 DNA detection of different vesicle fractions isolated by acoustofluidic device. B: HPV16 signals in different vesicle fractions. C: Results of HPV16 ddPCR assay for saliva exosomes of HPV-OPC patients. Eight of 10 patients (80%) were positive for HPV16 DNA. Dashed line shows a threshold of detection at 47.8 copies/mL saliva. Exo, exosomes; MV, microvesicles; NTC, negative control; PTC, positive control with synthetic HPV E7 gene.