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. 2020 Jan;190(1):48–56. doi: 10.1016/j.ajpath.2019.09.010

Figure 3.

Figure 3

Kv11.1 channels are expressed in healthy rat lungs. A: Hematoxylin and eosin (H&E) staining of Sprague-Dawley rat lung tissue showing a normal structure of a bronchiole (b), alveoli (a), thin interstitial alveolar wall, and normal pulmonary arteries wall thickness (pv; arrows) in control rats. B: α-Smooth muscle actin antibody staining in the smooth muscle cell layer of a bronchiole (asterisk) and in the media of pulmonary arteries (PAs) at diameter >100 μm (black arrow). Absence of α-smooth muscle actin antibody staining in the media of PAs at diameter <100 mm (red arrows). C: Kv11.1 antibody staining in bronchial SMC layer (asterisk) and in the media of PAs at diameter >100 μm (black arrow). Images in ×400 magnification illustrate the absence of Kv11.1 antibody staining in small PAs (red arrows). D: Quantification of Kv11.1 channel expression in large and small PAs. E: Negative control for C with the primary antibody omitted and only the secondary antibody used. The mean of the data is indicated (red lines). n = 11 per group. *P < 0.05. Scale bars: 100 μm (A–C, right panels); 200 μm (A–C, left panels, E). Original magnification: ×200 (A–C, left panels); and ×400 (A–C, right panels). IHC, immunohistochemistry.