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. 2020 Jan;190(1):48–56. doi: 10.1016/j.ajpath.2019.09.010

Figure 4.

Figure 4

Kv11.1 channel expression increases in pulmonary arterial hypertension (PAH) rats. A: Hematoxylin and eosin (H&E) staining of lung tissue from Sprague-Dawley rats with PAH shows pulmonary arteries (PAs) wall thickness (arrows). After SU5416 injection, rats were kept in hypoxia for 3 weeks and then maintained in normoxia for 5 weeks. B: α-Smooth muscle actin (SMA) antibody staining in the media of small PAs (brown; arrows). C: Kv11.1 antibody staining in the media of small PAs (brown; arrows). D: Quantification of Kv11.1 channel expression (in arbitrary units; au) in small PAs of healthy and PAH rat lungs. E: Negative control for C with the primary antibody omitted and only the secondary antibody used. The mean of the data is indicated (red lines). n = 11 (control); n = 18 (PAH). *P < 0.05. Scale bars = 100 μm (A–C and E). Original magnification, ×400 (A–C and E). IHC, immunohistochemistry.