Effect of Neu-shRNA on invasive and migratory ability of BGC823 cells. A. The expression of HER2 in gastric cancer cells BGC823, MKN45, and SGC7901. The expression of HER2 is higher in BCG823 cells compared with the other two cell lines. We chose BCG823 cells for HER2 knock-down test. B, C. Cells migration and invasion ability were evaluated by trans-well chambers. The cells were counted in 5 random fields under a light microscope (magnification, 200). Compared with BGC823 and shRNA-BGC823 cells, the migration and invasion ability were decreased in HER2 knocked-down Neu-shRNA-BGC823 cells. D. Cells migration were evaluated by wound healing (magnification, 200). P<0.01. Cell migration ability was decreased in Neu-shRNA-BGC823 cells compared with control groups.