Figure 2.

Effect of HER-2 on invasive and migratory abilities of MKN45 cells. A. Cells migration were evaluated by trans-well chambers. Compared with untreated MKN45 cells and PCDNA3-MKN45 cells, migration and invasion abilities were dramatically increased in HER2 up-regulated MKN45 cells. B. Cells migration were evaluated by scratch assay (magnification, 200). P<0.01. The results were consistent with trans-well chambers. C. Cells invasion were evaluated by Matrigel-coated trans-well chambers. The cells were counted in 5 random fields under a light microscope (magnification, 200). Compared with control groups, cell invasion was increased in HER2(CA)-MKN45 and HER2(WT)-MKN45 cells.