Figure 3.

The effect of irisin on adipogenic differentiation. A. The human visceral preadipocytes were induced to adipogenic differentiation with or without irisin (50 nM) for 18 days. Cells were stained with Oil Red O to visualize lipid droplets (left); mature adipocytes were calculated as percentage of control (right). B. Expression of adipogenic genes (CEBPB and ADIPOQ) and browning genes (PGC1α and PRDM16) in differentiated adipocytes were determined by qRT-PCR. C. mRNA expression levels of adipogenic genes (PPARR and ADIPOQ) were measured in vcWAT after 4 days of irisin (50 nM) treatment. Data are expressed as mean ± SEM; *P<0.05, **P<0.01 and ***P<0.001 statistical differenced compared to control.