Figure 4.

The effect of irisin on osteogenic differentiation. (A) The human visceral preadipocytes were induced to osteogenic differentiation with or without irisin (50 nM) for 21 days, as evidenced by mineral deposition highlighted by Alizarin Red staining. (B) mRNA expression levels of RUNX, OSTERIX and OPN genes were measured in fully differentiated visceral adipocytes (21 days) and (C) in vcWAT after 4 days treatment with irisin directly. Data are expressed as mean ± SEM; * P<0.05, **P<0.01 and ***P<0.001 statistical differenced compared to control.