Skip to main content
. 2019 Dec 15;11(12):7385–7397.

Figure 2.

Figure 2

MiR-510 overexpression alleviated the inflammatory injury induced by LPS in Caco-2 cells. Different concentrations of LPS (0, 1, 5, and 10 μg/ml) were used to stimulate cells for 5 h, and inflammatory injury was observed in Caco-2 cells. At LPS concentrations of 5 and 10 μg/ml, (A) CCK8 assay showed that cell viability was significantly decreased, and (B) flow cytometry showed that cell apoptosis was dramatically increased in Caco-2 cells. (C) mRNA levels and (D) concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) in the culture supernatant were significantly upregulated in LPS-treated Caco-2 cells compared with the control according to qRT-PCR and ELISA. The inflammatory injury induced by LPS was rescued by miR-510 overexpression. (E) Cell viability was dramatically increased. (F) Cell apoptosis and (G) mRNA expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) were significantly decreased, and the concentrations of pro-inflammatory cytokines in the culture supernatants, such as (H) TNFα, (I) IL-1β, (J) IL-6, and (K) IL-8, were significantly decreased. *: P<0.05. **: P<0.01, ***: P<0.001.