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. 2019 Dec 15;11(12):7385–7397.

Figure 3.

Figure 3

MiR-510 negatively regulated PRDX1 expression in Caco-2 cells by binding to the 3’-UTR of PRDX1 directly. (A) Bioinformatics analyses showed that the PRDX1 3’-UTR is a potential target of miR-510. (B) Dual-luciferase reporter assay exhibited dramatic reduction of luciferase activity in HEK293 cells co-transfected with miR-510 mimics and pGL3-PRDX1-3’-UTR-WT compared with the cells co-transfected with miR-510 mimics and pGL3-PRDX1-3’-UTR-MUT. (C) qRT-PCR and (D) Western blots showed that PRDX1 was downregulated in the miR-510 mimic group but was upregulated in the miR-510 inhibitor group. *: P<0.05. **: P<0.01.