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. 2019 Dec 15;11(12):7555–7567.

Figure 3.

Figure 3

The HUVECs group heat treated of 45°C for 60 minutes was used for subablative treatment. (A) The viability of HUVECs was significantly lower after heat treatment of 45°C for different times. Cell viability was approximately 50% after 60 minutes of heat treatment. (B) LDH release after 60 minutes of heat treatment was significantly higher than those of after 15, 30, 45 minutes of heat treatment respectively. (C-G) The supernatant levels of HMGB1, IL-1β, IL-18, LDH and caspase-1 of each group were measured y the method of ELISA. Supernatant evels of (C) HMGB1, (D) L-1β, (E) IL-18 (F) LDH, and (G) caspase-1 increased significantly after subablative treatment and were reduced obviously when EP was added. *P < 0.05. **P < 0.01.