Figure 6.

Effects of As^III and Tetra, each alone or in combination, on the population of CD4⁺ T cells and Treg cells in mitogen-activated human PBMCs. After treatment with 0.5 μM As^III and 0.5 μg/ml Tetra, alone or in combination, for 72 h, alterations of CD4⁺ T cell and Treg cell populations in ConA-activated PBMCs were analyzed by a flow cytometer as described in materials and methods. (A) Lymphocyte fraction of PBMCs (a1) were gated for CD4⁺ T cells (a2), and the percentage of Treg cells in the CD4⁺ T cells fraction in each treatment were calculated (B). Treg cells (C) and CD4⁺ T cells (D) in ConA-activated PBMCs treated with 0.5 μM As^III and 0.5 μg/ml Tetra, alone or in combination, for 72 h were calculated, respectively. Data are presented as means ± SD from more than three independent experiments. As, As^III; Tetra, tetrandrine.