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. 2019 Dec 13;24(24):4578. doi: 10.3390/molecules24244578

Table 4.

Experimental factors for the two-level fractional factorial experimental (one-half).

Factor Description Low Level High Level
A pH a 3.00 3.80
B Ethanol (%) b 13.5 14.8
C Sugar (g/L) c 0.16 8.00
D Temperature (°C) 10 20
E Agitation No Yes

a Wine samples were measured for pH by using a Mettler Toledo T50 Autotitrator (Port Melbourne, VIC, Australia). b The ethanol concentration was determined with the Anton Paar Alcolyzer Wine ME and DMA 4500M (North Ryde, NSW, Australia). c The sugar content were determined via Chemwell® 2910 Automated EIA and Chemistry Analyser (Awareness Technology, Palm City, FL, USA) with the Megazyme K-FRUGL test kits (Chicago, Illinois, USA).