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Effects of APR TD012 on NRF2 and its target expressions. Immunoblot analysis of ATP2C1, Notch1 Val1744 (A) and NRF2 (B) in siCTR and siATP2C1 HaCaT cells. Tubulin expression was used as control for equal loading. qRT-PCR analysis of NQO1 mRNA expression levels in siCTR and siATP2C1 HaCaT (C) or NHEK (D) cells. The values are expressed as fold changes of siATP2C1 cells vs. the siCTR. The averages ± standard error of two independent experiments are shown.
