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. 2019 Dec 15;24(24):4592. doi: 10.3390/molecules24244592

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) The localization of DAF-16::GFP under fluorescent irradiation. Under normal growth conditions, DAF-16 is mainly retained in the cytoplasm. When daf-16 was expressed in the nucleus, the fluorescence signal of DAF-16::GFP was gathered in the nucleus, and the whole nematode was observed under the microscope with a spot-like green fluorescence. (b) The number of fluorescence points of DAF-16::GFP under the oxidative stress state of paraquat. The number of fluorescence points represented the amount of expression of daf-16 into the nucleus. *** p < 0.001, NPRR vs. Blank; ** p < 0.01, NPRRP vs. Blank.