Figure 1.

The surface reactivity of glutathione-s-transferase omega 2 (GSTO2) on non-permeabilized mouse and boar spermatozoa using indirect immunofluorescence. Non-permeabilized fresh boar and mouse spermatozoa were stained using a GSTO2 specific (Y-12) antibody (GSTO2), or a GSTO-specific fluorescent inhibitor (Inhibitor). Nuclei were labelled with DAPI. To confirm the specificity of the antibody, the anti-GSTO2 antibody was preincubated with its blocking agent (blocking peptide, the peptide used to raise the antibody) according to the manufacturer’s instructions and a secondary-only control was done (Secondary Only). The GSTO inhibitor is a membrane permeable cell tracker probe (Invitrogen Molecular Probe C7025). The bar represents 10 μm.