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. 2019 Nov 29;8(12):601. doi: 10.3390/antiox8120601

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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GSTO2 reactivity to biotin-isolated surface proteins of ejaculated and capacitated boar spermatozoa. Immunoblotting using an anti-GSTO2 (Y-12) antibody on biotin-isolated surface proteins from ejaculated boar sperm (Lane 1) and capacitated boar sperm (Lane 2). The antibody was pre-incubated with its corresponding blocking peptide to show specificity in both the ejaculated boar sperm (Lane 3) and capacitated boar sperm (Lane 4) samples.