Figure 5.

Sperm capacitation is reduced by the presence of the GSTO inhibitor when incubated in a capacitation medium. Sperm treatment was done through incubation for 25 min before in vitro capacitation was induced. The acrosome exocytosis reaction (a hallmark of capacitation) was induced using progesterone after 60 min of capacitation. Sperm samples were fixed and stained with PNA-647 and 4’,6-diamidino-2-phenylindole (DAPI) and scored based on acrosome labelling. At least 200 sperm per treatment were assessed per trial and grouped into acrosome intact or acrosome reacting/reacted. Statistical significance was determined by multiple t-tests and is denoted by *, p = 0.0004.