Figure 6.

Computer-aided sperm analysis (CASA) of GSTO-inhibited and DMSO treated (Control) mouse spermatozoa after in vitro capacitation. Sperm motility parameters were analyzed on capacitated mouse spermatozoa that were either treated with DMSO (Control) or GSTO inhibitor prior to a 60-min incubation in capacitating medium at 37 degrees Celsius. The average total motility (A) was the combination of the progressive and non-progressive motility scores for each sample, whereas (B) shows solely the comparison of progressive motility. Both the total and progressive motility differences between the two treatment groups were statistically significant. The curvilinear velocity of GSTO inhibited sperm were also significantly decreased compared to controls when both treatment groups were capacitated (C). A higher linearity was also observed when spermatozoa were treated with the GSTO inhibitor (D), but the differences between the control and inhibited treatments were not found to be statistically significant. Error bars represent standard error. Statistical significance was determined using multiple t-tests and is denoted by *.