Figure 7.

The assessment of sperm membrane lipid peroxidation after in vitro capacitation in mouse spermatozoa. (A) Lipid peroxidation levels were assessed based on the red and green fluorescence intensities of cells treated with the BODIPY 581/591 C11 probe. The relative intensity was calculated as the intensity of the green fluorescence over total fluorescence intensity. Spermatozoa were treated with the BODIPY 581/591 C11 probe and then incubated with either the GSTO Inhibitor or DMSO (Control) for 25 min before in vitro capacitation. After 60 min of in vitro capacitation, samples were fixed and imaged to determine the red and green fluorescence intensities of each cell. At least 200 cells were individually imaged in each treatment group for each trial, and a representative image from the control and GSTO inhibited groups is shown in (B). Three trials were performed, each with different mice, and error bars represent standard error. Statistical significance was determined using a t-test with Welch’s correction and is denoted by *.