FIGURE 1.

Tissue and cellular distribution of S1P lyase in mice with chronic ileitis. A, SPL mRNA transcripts (Sgpl1) were assessed by real-time RTPCR in indicated tissues of 10-week-old TNFΔARE mice with established ileitis. B, SPL mRNA expression in epithelial cells, lamina propria leukocyte (LPL) fractions, and whole ileal tissue relative to GAPDH. C, Tissue and plasma S1P levels were measured using the LC-MS platform. D, Localization of the SPL protein in indicated tissues was assessed by immunohistochemistry (representative images). Data are expressed as mean ± SEM, n ≥ 4 mice/group (*P < 0.05).