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. 2019 Dec 6;26(2):216–228. doi: 10.1093/ibd/izz174

FIGURE 6.

FIGURE 6.

S1P lyase inhibition did not induce leukocyte apoptosis in the intestine in mice with chronic ileitis. A, Annexin V staining total CD4+ and CD8+ T cells isolated from TNFΔARE mice treated with DOP or vehicle (control) for the indicated number of days. Data are expressed as mean ± SEM; n ≥ 4 mice/group/time point. B, Caspase 3 mRNA transcripts relative to GAPDH as quantified by real time Q-PCR after 2 weeks of DOP treatment. Data are expressed as mean ± SEM, n ≥ 9 mice/group. C, Sphingosine levels in the ileum of DOP- and vehicle-treated mice. Data are expressed as mean ± SEM; n ≥ 9 mice/group (**P < 0.01 by 2-tailed t test). D, TUNEL staining of the ileum of TNFΔARE mice treated for 2 weeks with DOP and vehicle control. Representative images are from 3 independent experiments. Scale bar = 100 μm.