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. 2019 Dec 6;24(24):4482. doi: 10.3390/molecules24244482

Figure 3.

Figure 3

(a) LCMS-UV Chromatogram at 260 nm and (b) deconvoluted mass of B (calculated mass 5361.03, found mass 5361.59). (c) Overlay of the HPLC runs of the unmodified (black line) and labeled RNAs A (red line) and B (blue line). (d) Experimental cw EPR spectra of 35 µM A2 (red line) and 35 µM B2 (blue line). (e) Thermal denaturation curves of the unmodified (black line, Tm = 66 °C) and labeled RNAs A2 (red line, Tm = 58 °C) and B2 (blue line, Tm = 55 °C). (f) Circular Dichroism (CD) spectra of the unmodified (black line) and labeled RNAs A2 (red line) and B2 (blue line).