Figure 1.

Exercise training prevents high-fat diet (HFD)-induced obesity in mice. (A) Timeline for diet and exercise training plan. Mice were randomly chosen for 20 weeks of high-fat diet (HFD) and exercise training (EX) at 10 weeks of age. All timepoints are reported as weeks of treatment starting at 10 weeks of age. (B) Average weekly body weight in the four groups of mice: normal diet (ND), HFD, HFD mice on exercise training (HFDEX), and ND mice on exercise training (NDEX). N = 8/group. (C) Food consumption over a two-week period was unchanged between groups. (D) Total calorie intake was increased in HFD-treated mice. (E) Representative DEXA imaging of mice after 18 weeks of diet and exercise training regimen. (F) Quantification of body fat percentage from DEXA. HFD treatment increased body fat percentage, which was prevented by exercise training. All values are expressed as mean ± SEM with dots or triangles representing each animal. One-way analysis of variance (ANOVA) and Tukey’s post-hoc test were used for statistical analysis. Repeated measures of ANOVA were used for B. *P < 0.05 between ND and HFD.