Fig. 7.
Physiological and physicochemical parameters of hydrated thalli from M. tessellata (dark green) and P. crispa (light green) subjected to controlled freezing. (A) Kinetics of Fv/Fm during freezing (incubation at −20 ºC) and subsequent thawing at +20 ºC. Data points are average ± s.e. (n = 6). (B) De-epoxidation level of xanthophyll cycle before and after 48 h of incubation (n = 3). (C) Freezing temperature ranges obtained from onset temperatures of exothermic peak recorded with differential scanning calorimetry during cooling scan (n = 6; see Supplementary Data Fig. S5 for representative examples of the scans). When significant, differences between P. crispa and M. tessellata are depicted with an asterisk (P < 0.05).