Fig. 2.

Moderate but not heavy-intensity or long-time exercise induced tissue hypoxia in the kidney, liver, and colon. (A) Pimonidazole HCl was used to detect the tissue hypoxia in the kidney, liver, and colon of ODD-Luc mice. We found increased immunostaining in the kidney, liver, and colon of the ME group but not in the HE or LE group, compared to the SED group; The arrows show the areas of the renal tubules, the central veins and the crypt, which showed positive staining after 1 session of moderate exercise. (B) The MOD indicated the intensity of staining in specifically immunoreactive areas of the kidney, liver, and colon in the SED, ME, HE, and LE groups. n = 6/group; Scale bars = 100 μm. **p < 0.01. HC1 = hydrochloride; HE = heavy-intensity exercise; LE = long-time exercise; ME = moderate exercise; MOD = mean optical density; ODD = oxygen-dependent degradation domain; SED = sedentary control.