Fig. 3.

Exercise did not induce hypoxia in the heart, skeletal muscle or spleen tissue. Pimonidazole HCl was used to detect the tissue hypoxia. No positive staining was observed in the heart, skeletal muscle, and spleen of the ODD-Luc mice with or without exercise intervention. n = 6/group; Scale bars = 100 μm. HCl = hydrochloride; HE = heavy-intensity exercise; LE = long-time exercise; ME = moderate exercise; ODD = oxygen-dependent degradation domain; SED = sedentary control.