Fig. 4.

Exercise increased HIF-1α in the abdominal area. Bioluminescent imaging was used to detect HIF-1α expression in vivo. Bioluminescent imaging was used to detect HIF-1α expression in vivo. (A) Horizontal bars show the time-points of experiments. (B) Photons in the abdominal area of mice (left). FVB = FVBN/J mice were exposed to normoxic conditions, n = 3/group; CON = ODD-Luc mice were exposed to normoxic conditions, n = 3/group; 9% O₂ = ODD-Luc mice were exposed to 9% O₂ for 4 h, n = 3/group; SWIM = ODD-Luc mice swam for 30 min, n = 5/group; SWIM+PX-478 = ODD-Luc mice injected with PX-478 and swam for 30 min, n = 3/group. The average photon radiance of the region of interest was also calculated (right). (C) Photons in the abdominal area of mice that underwent different exercise interventions (left). FVB = FVBN/J mice were exposed to normoxic conditions, n = 3/group; CON = ODD-Luc mice were exposed to normoxic conditions, n = 3/group; ME = ODD-Luc mice swam for 30 min, n = 4/group; HE = heavy-intensity exercise of ODD-Luc mice, n = 3/group; LE = long-time exercise of ODD-Luc mice, n = 4/group. The average photon radiance of the region of interest was also calculated (right). The color bar indicates the photon level (cm²/s/steradian) and the minimum and maximum threshold values. *p < 0.05. HE = heavy-intensity exercise; HIF = hypoxia-inducible factor; LE = long-time exercise; ME = moderate exercise; ODD = oxygen-dependent degradation domain.