Fig. 6.

The post-exercise HIF-1α level changed in a time-dependent manner. Bioluminescent imaging was applied to detect HIF-1α expression in vivo. (A) Horizontal bars show the time-points of detection in the SWIM group. (B) The photon levels in the abdominal area of ODD-Luc mice were recorded at the 0th, 2nd, 4th, 6th, 8th, 12th, 16th, 20th, and 24th h after a moderate exercise bout of swimming. 0th h: n = 4/group; 2nd h: n = 5/group; 4th h: n = 4/group; 6th h: n = 4/group; 8th h: n = 3/group; 12th h: n = 3/group; 16th h: n = 3/group; 20th h: n = 3/group; 24th h: n = 3/group. The scattergram indicates the individual expression of HIF-1α at different time-points. Luciferase activity was increased at the 0th h post-exercise and gradually returned to the baseline value during the next 24 h. The color bar indicates the photon level (cm²/s/steradian) and minimum and maximum threshold values. *p < 0.05. CON = ODD-Luc mice were exposed to normoxic conditions; FVB = FVBN/J mice were exposed to normoxic conditions; HIF = hypoxia-inducible factor; ODD = oxygen-dependent degradation domain.