Skip to main content
. 2020 Jan;190(1):176–189. doi: 10.1016/j.ajpath.2019.09.013

Figure 7.

Figure 7

Treatment with the senolytic agent FOXO4-DRI does not significantly affect tubular cell senescence but decreases apoptosis in Glis2 knockout kidneys. A: Representative bright-field microscopy images of kidney sections showing senescence-associated β galactosidase (SA-β-Gal) activity in Glis2 knockout mice treated with FOXO4-DRI of vehicle, at low (top row) and high (bottom row) magnification. B: Quantification of staining in A by digital image analysis. C–H: Representative immunofluorescence confocal images for kidney sections from both genotypes, after immunostaining for: the proliferation marker Ki-67 (C), with the corresponding quantification (D); the DNA damage marker γ-H2AX (E), with the corresponding quantification (F); and the apoptosis marker activated caspase 3 (G), with the corresponding quantification (H). P values were calculated using the two-tailed t-test. Numbers of mice per group are reported in the panels. Data are expressed as means ± SD (B, D, F, and H). *P < 0.05, ***P < 0.001. Scale bars: 1 mm (A, top row); 200 μm (A, bottom row); 20 μm (C, E, and G).