Supplemental Figure S5.

Representative images of Ki-67, KRT14, and KRT5 coimmunostaining in keratinocyte growth factor (KGF)–pretreated bladders 6 hours after cyclophosphamide injection. Triple-label immunofluorescence for Ki-67 (red), KRT14 (white), and KRT5 (green). A and D: Ki-67 staining shows no Ki-67⁺ (proliferating) cells in PBS-pretreated urothelium (A), but several Ki-67⁺ proliferating cells in the KGF-pretreated urothelium (arrow) 6 hours after cyclophosphamide injection. A, B, D, and E: Ki-67 and KRT14 merged staining shows rare KRT14⁺ cells in the basal layers of both PBS-pretreated mice (arrowheads in A and B) and KGF-pretreated mice (arrowheads in D and E), none of which are Ki-67⁺. Dotted lines indicate demarcation between urothelial layer and underlying lamina propria. C, E, and F: Ki-67 and KRT5 merged staining shows that the rare KRT14⁺ cells are also KRT5⁺ in both groups (arrowheads; C and F) and that the Ki-67⁺ proliferating cells in the KGF group are all KRT5⁺/KRT14⁻ (arrows; E and F). A and D: Blue indicates DAPI. Scale bar = 50 μm (A–F). L, lumen.