Supplemental Figure S6.

Representative images of phosphorylated AKT (pAKT) and Ki-67 coimmunostaining in keratinocyte growth factor (KGF)–pretreated bladders 6 hours after cyclophosphamide injection. A: Immunostaining for pAKT (green) and Ki-67 (red) reveals limited expression in PBS-pretreated bladders 6 hours after cyclophosphamide administration. B: In comparison to PBS-pretreated/injured mice, KGF-pretreated/injured bladders have increased and diffuse pAKT staining across most of the urothelium, including in most of the predominantly Ki-67⁻ (arrow) and in some of the Ki-67⁺ (concave arrowhead) cells, although some Ki-67⁺ cells do not express pAKT (arrowhead). Blue indicates DAPI. Dotted lines indicate demarcation between urothelial layer and underlying lamina propria. Scale bar = 50 μm (A and B). L, lumen.