Supplemental Figure S9.

Representative images of Ki-67 and KRT14 coimmunostaining in keratinocyte growth factor (KGF)–pretreated bladders at 6 hours, 1 day, and 3 days after sham injury. Merged images of double-label immunofluorescence for Ki-67 (red) and KRT14 (white) show the presence of few KRT14⁺ cells (arrowheads) in the basal layer with either PBS pretreatment (A, C, and E) or KGF pretreatment (B, D, and F) at 6 hours (A and B), 1 day (C and D), or 3 days (E and F) after sham injury. None of the KRT14⁺ cells in any group is Ki-67⁺. Dotted lines indicate demarcation between urothelial layer and underlying lamina propria. Scale bar = 50 μm (A–F). L, lumen.