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. Author manuscript; available in PMC: 2020 Jan 6.
Published in final edited form as: Anal Chem. 2018 Apr 3;90(8):5171–5178. doi: 10.1021/acs.analchem.7b05304

Figure 2.

Figure 2

Sample electropherograms and calibration curve. (A) Sample electropherograms at 0 nM and 200 nM insulin standards. Bound and free indicate Ab:Ag* complex and free Ag*, respectively. There are 2 free peaks because FITC-insulin are mono- and double-labeled. We use the first free peak area for B/F calculation. B/F is approximately one at 0 nM insulin. (B) A sample calibration curve. Error bars indicate ±1 standard deviation. The mean and standard deviation were calculated based on 10–15 electropherograms for each data point.