Figure 3.

Regulatory role of IRAK-M deficiency in the activities of bone marrow–derived dendritic cells (BMDCs). (A) Flow cytometry analysis of expression of costimulatory molecules on lung DCs from PBS- or OVA-treated WT and IRAK-M KO mice. n = 10–15 mice in each group; *P < 0.05. (B) In vitro flow cytometry analysis of the expression of the costimulatory molecule OX40L on IRAK-M KO or WT BMDCs at baseline or after OVA stimulation; n = 10 in each group; *P < 0.05. (C) ELISA of cytokine production by IRAK-M KO or WT BMDCs after in vitro OVA stimulation. n = 10–15 in each group; *P < 0.05. (D) In vitro differentiation of naive CD4⁺ T cells incubated with IRAK-M KO or WT BMDCs, with the presence of the indicated costimulatory factors. The percentages of T cell subpopulations were detected by flow cytometry analysis and plotted. n = 10 in each group; *P < 0.05. (E) Flow cytometry analysis of intracellular transcription factors, including p-IκBζ, IκBζ, phosphorylated c-JUN N-terminal protein kinase (p-JNK), and p-p38 mitogen-activated protein kinase (MAPK), in IRAK-M KO or WT BMDCs after in vitro OVA stimulation. n = 10 in each group; *P < 0.05. MFI, mean fluorescent intensity.