Fig. 5.

Two substitutions affected viral replication, transcription and translation. a Virus RNA level in A549 cells at 6 h post-infection was investigated. Levels of M genes were estimated by qRT-PCR and normalized with the β-actin genes of cells. Negative-sense viral vRNA and positive sense viral mRNA were compared. The one-way ANOVA with Tukey post-test was performed; *p < 0.05, **p < 0.01. b Virus NP protein levels in A549 cells at 9 h post-infection were examined. The expression level of NP and β-actin were examined by Western blot and analyzed by Quantity One software