Fig. 7.

Validation of COL1A1 gene expression by IHC and qRT-PCR and a predictor for 1-year survival from HF onset. a The demonstrations of IHC results of normal controls and HF patients, the upper is from a region of LV from a normal control and the under is from a HF patient. b IHC staining of COL1A1 shows proportion of COL1A1-positive area of whole slice in HF is higher than normal control. c qRT-PCR results of COL1A1 gene expression in left ventricles of normal and HF hearts. d Plasma COL1A1 levels could distinguish the 1-year HTx from non-HTx when using cutoff value 256.5 ng/ml (AUC = 0.789, p value < 1.0 × 10^− 4). e COL1A1 level ≥ 256.5 ng/ml was statistically associated with poor survival