Fig. 4.

PTEN expression, regulated via the SNHG1/miR-153-3p signaling axis, inhibits viability and promotes apoptosis in SH-SY5Y cells treated with MPP⁺. a Presumed binding sites of miR-153-3p in the wild-type or mutant PTEN3′-UTR. b Luciferase activity of SH-SY5Y cells transfected with PTEN-wt or PTEN-mut reporter and miR-NC, miR-153-3p, miR153-3p + pcDNA or miR-153-3p + pcDNA-SNHG1 was determined by luciferase reporter assay. c Western blot assays were performed to assess the effects of miR-153-3p overexpression or SNHG1 knockdown on PTEN protein expression. Expression levels of PTEN protein in the midbrain of MPTP-induced PD mice d and MPP⁺-treated SH-SY5Y cells e were determined by western blotting. f Expression level of PTEN protein in SH-SY5Y cells after transfection with si-PTEN or pcDNA-PTEN. g, h Effect of PTEN overexpression or silencing on cell viability and apoptosis in the presence of MPP⁺. *P <0.05; **P < 0.01