Table 1.
This table demonstrates differential RNA-based gene expression, in linear fold change, for various immune and/or cytotoxic relevant genes, as compared to control. Several genes including HSP70, MDM2, Gadd45 and CXCL10, were dramatically altered following low dose (CEM 30) mNPH.
| Gene | mNPH | 8 Gy | mNPH 8 Gy |
|---|---|---|---|
| ICAM-1 | .766 | 1.53 | 15.7* |
| CD86 | 2.09 | 1.32 | 29.1* |
| CXCL10 | 2.6* | .657 | 2.83* |
| CXCL11 | 1.18* | .713 | 4.28* |
| CXCR3 | 1.39 | 1.31 | 8.86* |
| TLR4 | .81 | .864 | .457 |
| TLR3 | .728 | .848 | .24* |
| ERK1 (MAPK3) | .478* | 1.06 | .402* |
| ERK2 (MAPK1) | .837 | .882 | .564* |
| MAPK11 | .619 | .838 | .2* |
| MAPK14 | 1.15 | .85 | .842 |
| Bcl-2 | 1.53 | 1.19 | .982 |
| Bax | 1.38 | 1.46* | 1.14 |
| PUMA (bbc3) | 1.04 | 1.53 | 4.75* |
| CASP8 | .687* | .893 | .572* |
| CASP3 | .638 | .976 | .343* |
| Gadd45 | 3.38* | 1.21 | 4.65* |
| Bim (bcl2l11) | .481 | 1.09 | .364* |
| survivin | .373 | .923 | .1* |
| mTOR | .902 | .898 | .781 |
| HSP70 (hspab1) | 57* | .884 | 41.8* |
| MDM2 | 3.61* | 1.65* | 3.12* |
| Fas | 2.08* | 1.54 | 6.85* |
These expression changes were amplified with the addition of radiation. Other notable genes such as the pro-apoptotic gene bbc3 (PUMA) were altered mostly by the combination treatment.
*
Statistically significant at p < .05, differential expression.